Ah sorry, misread your query; you want this:
INSERT INTO tblUserTeams (TeamName, LeaderCode, LeaderDiscipline, MemberCode, MemberDiscipline)
VALUES ('TEAM_C','SQU01', 'PT',
LEFT('WIL01-JACK WILLS, PTA',(CHARINDEX('-','WIL01-JACK WILLS, PTA') - 1)),
SUBSTRING('WIL01-JACK WILLS, PTA',CHARINDEX(',','WIL01-JACK WILLS, PTA')+1,LEN('WIL01-JACK WILLS, PTA')-CHARINDEX(',','WIL01-JACK WILLS, PTA')))