Yes, of course you can do. I recommend to change the settings on one machine for an arbitrary profile, export the reg entries, remove unwanted changes, and modify them to apply to the .DEFAULT user. Applying to existing users is more complicated if they are not cloned ones (having the same user SID string as seen under the HKEY_USERS hive).
The related registry settings are in:
HKCU\Control Panel\International
HKCU\Control Panels\Keyboard Layout
HKLM\System\CurrentControlSet\Control\TimeZoneInformation.
You can export them one by one, and then merge the results into a single REG file.