Question : Login failed for user ''. The user is not associated with a trusted SQL Server connection.

I have developed  a asp page. Once I try to test my application I get this page. I'm connected to a test database. I was wondering if someone can help me resolve this?
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Source Error: 

An unhandled exception was generated during the execution of the current web request. Information regarding the origin and location of the exception can be identified using the exception stack trace below.  

Stack Trace: 


[SqlException (0x80131904): Login failed for user ''. The user is not associated with a trusted SQL Server connection.]
   System.Data.SqlClient.SqlInternalConnection.OnError(SqlException exception, Boolean breakConnection) +4846887
   System.Data.SqlClient.TdsParser.ThrowExceptionAndWarning(TdsParserStateObject stateObj) +194
   System.Data.SqlClient.TdsParser.Run(RunBehavior runBehavior, SqlCommand cmdHandler, SqlDataReader dataStream, BulkCopySimpleResultSet bulkCopyHandler, TdsParserStateObject stateObj) +2392
   System.Data.SqlClient.SqlInternalConnectionTds.CompleteLogin(Boolean enlistOK) +35
   System.Data.SqlClient.SqlInternalConnectionTds.AttemptOneLogin(ServerInfo serverInfo, String newPassword, Boolean ignoreSniOpenTimeout, Int64 timerExpire, SqlConnection owningObject) +144
   System.Data.SqlClient.SqlInternalConnectionTds.LoginNoFailover(String host, String newPassword, Boolean redirectedUserInstance, SqlConnection owningObject, SqlConnectionString connectionOptions, Int64 timerStart) +342
   System.Data.SqlClient.SqlInternalConnectionTds.OpenLoginEnlist(SqlConnection owningObject, SqlConnectionString connectionOptions, String newPassword, Boolean redirectedUserInstance) +221
   System.Data.SqlClient.SqlInternalConnectionTds..ctor(DbConnectionPoolIdentity identity, SqlConnectionString connectionOptions, Object providerInfo, String newPassword, SqlConnection owningObject, Boolean redirectedUserInstance) +189
   System.Data.SqlClient.SqlConnectionFactory.CreateConnection(DbConnectionOptions options, Object poolGroupProviderInfo, DbConnectionPool pool, DbConnection owningConnection) +185
   System.Data.ProviderBase.DbConnectionFactory.CreatePooledConnection(DbConnection owningConnection, DbConnectionPool pool, DbConnectionOptions options) +31
   System.Data.ProviderBase.DbConnectionPool.CreateObject(DbConnection owningObject) +433
   System.Data.ProviderBase.DbConnectionPool.UserCreateRequest(DbConnection owningObject) +66
   System.Data.ProviderBase.DbConnectionPool.GetConnection(DbConnection owningObject) +499
   System.Data.ProviderBase.DbConnectionFactory.GetConnection(DbConnection owningConnection) +65
   System.Data.ProviderBase.DbConnectionClosed.OpenConnection(DbConnection outerConnection, DbConnectionFactory connectionFactory) +117
   System.Data.SqlClient.SqlConnection.Open() +122
   System.Data.Common.DbDataAdapter.QuietOpen(IDbConnection connection, ConnectionState& originalState) +31
   System.Data.Common.DbDataAdapter.FillInternal(DataSet dataset, DataTable[] datatables, Int32 startRecord, Int32 maxRecords, String srcTable, IDbCommand command, CommandBehavior behavior) +112
   System.Data.Common.DbDataAdapter.Fill(DataSet dataSet, Int32 startRecord, Int32 maxRecords, String srcTable, IDbCommand command, CommandBehavior behavior) +287
   System.Data.Common.DbDataAdapter.Fill(DataSet dataSet, String srcTable) +92
   System.Web.UI.WebControls.SqlDataSourceView.ExecuteSelect(DataSourceSelectArguments arguments) +1297
   System.Web.UI.WebControls.ListControl.OnDataBinding(EventArgs e) +95
   System.Web.UI.WebControls.ListControl.PerformSelect() +34
   System.Web.UI.WebControls.BaseDataBoundControl.DataBind() +73
   System.Web.UI.WebControls.BaseDataBoundControl.EnsureDataBound() +82
   System.Web.UI.WebControls.BaseDataBoundControl.OnPreRender(EventArgs e) +22
   System.Web.UI.WebControls.ListControl.OnPreRender(EventArgs e) +18
   System.Web.UI.Control.PreRenderRecursiveInternal() +80
   System.Web.UI.Control.PreRenderRecursiveInternal() +171
   System.Web.UI.Control.PreRenderRecursiveInternal() +171
   System.Web.UI.Page.ProcessRequestMain(Boolean includeStagesBeforeAsyncPoint
 

Answer : Login failed for user ''. The user is not associated with a trusted SQL Server connection.

what account is the app pool used by this application configured for?

This article provides some other things to look for
http://blogs.msdn.com/b/sql_protocols/archive/2008/05/03/understanding-the-error-message-login-failed-for-user-the-user-is-not-associated-with-a-trusted-sql-server-connection.aspx
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